PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM PALMITIC ACID USING Ralstonia eutropha BY FED BATCH CULTURE
نویسندگان
چکیده
Polyhydroxyalkanoates (PHA’s) are carbon and energy storage compounds which accumulate intracellulary as granules in certain bacteria during unbalanced growth and in the presence of an excess carbon source. PHAs were well studied regarding their promising applications, physical, chemical and biological properties. PHAs are biodegradable, biocompatible, have good material properties, renewable and can be used in many applications. In this study, we have biosynthesized Poly(β-hydroxyalkanoate)s (PHAs) by Ralstonia eutropha (formerly known as Alcaligenes eutrophus) by using pamitic acid extracted from palm oils. The bacterium Ralstonia eutropha accumulates high levels of PHA and can effectively utilize Palmitic acid. The Ralstonia eutropha H 16 successfully incorporated Poly 3-hydroxyhexanoate (PHB) monomer when fed with crude palm kernel oil (CPKO) as the sole carbon source. The experiments were carried out through submerged discontinuous cultures in agitated flasks and a bioreactor. The cultures of R. eutropha were grown for 24h at 30oC. Approximately 63 ± 2 wt% of P(3HB-co3HHx) copolymer with 4 mol% of PHB was synthesized from 5 g/L of oil after 48 h of cultivation. The results showed that R. eutropha growth in this carbon source was similar when compared with the standard medium (μm = 0.20 h-1). R.Eutropha demonstrated its ability to synthesize P(3HB-co-3HHx) copolymer from a readily available and renewable carbon source; CPKO, limiting the Nitrogen component in a fermentor without the addition of any precursors.
منابع مشابه
Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture
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